Japanese encephalitis virus replication: studies on host cell nuclear involvement.

K R Leary; C D Blair

doi:10.1016/0014-4800(83)90091-6

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Japanese encephalitis virus replication: studies on host cell nuclear involvement.

K R Leary·C D Blair

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Abstract

Replication, as measured by virus production, of both the flavivirus Japanese encephalitis virus (JEV) and the alphavirus Venezuelan encephalitis virus (VEV) was unaffected by short pulses of actinomycin D (act D) at early times postinfection (PI). Replication of JEV was found to be partially inhibited by continuous exposure to act D under conditions where VEV replication was equally sensitive to the drug. JEV replication proceeded normally in the presence of mitomycin C, a DNA synthesis inhibitor. Autoradiographic analysis revealed that virus-specific RNA was present only in the cytoplasm at both early and late times PI. When infected cell membranes were separated on a discontinuous sucrose gradient, most of the virus-specific RNA was associated with the endoplasmic reticulum fraction.

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Animals Autoradiography Cell Fractionation Cell Line Cell Nucleus Cytoplasm Dactinomycin Encephalitis Virus, Japanese Endoplasmic Reticulum Mitomycin Mitomycins RNA, Viral Virus Replication Journal Article Research Support, U.S. Gov't, Non-P.H.S.Research Support, U.S. Gov't, P.H.S.

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K R Leary, C D Blair. 1983. Japanese encephalitis virus replication: studies on host cell nuclear involvement.. https://doi.org/10.1016/0014-4800(83)90091-6

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