Sulfhydryl reagents affect Na+ uptake into toad bladder membrane vesicles.

E F LaBelle; D C Eaton

doi:10.1007/bf01870673

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Sulfhydryl reagents affect Na+ uptake into toad bladder membrane vesicles.

E F LaBelle·D C Eaton

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Abstract

The effect of sulfhydryl reagents on the Na+ permeability mechanisms of toad urinary bladder vesicles was examined. The reagents 5,5'-dithiobis (2-nitrobenzoic acid) (DTNB), iodosobenzoate, and ethylenimine were able to decrease amiloride-inhibited sodium uptake into vesicles when used at low concentrations. When used at higher concentrations these reagents were able to induce large increases in vesicle Na+ permeability that were not sensitive to amiloride. The reagent p-chloro-mercuribenzene sulfonate was able to induce such leaks even at low concentrations. The reagent N-ethylmaleimide was incapable of substantially affecting vesicle Na+ transport in any way. All of the effects observed could be reversed by removing the reagents from the solution surrounding the vesicles. Our results help explain the varied actions of sulfhydryl reagents on intact epithelial tissue.

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Animals Biological Transport, Active Bufo marinus Cell Membrane Kinetics Sodium Sulfhydryl Reagents Urinary Bladder Journal Article Research Support, U.S. Gov't, P.H.S.

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E F LaBelle, D C Eaton. 1983. Sulfhydryl reagents affect Na+ uptake into toad bladder membrane vesicles.. https://doi.org/10.1007/bf01870673

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