Detection of IgD 'hidden' lambda light chain.

G Spira; I Silvian; I Tatarsky; A Carter

doi:10.1016/0022-1759(83)90348-4

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Detection of IgD 'hidden' lambda light chain.

G Spira·I Silvian·I Tatarsky·A Carter

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Abstract

Immunoelectrophoresis and immunodiffusion of some IgD myeloma proteins fail to demonstrate precipitin bands between the light chain and anti-light chain serum. Reduction and alkylation of purified IgD preparation was found to render the molecule reactive to anti-lambda serum. Iodinated IgD myeloma protein was successfully precipitated by both anti-delta and anti-lambda antibodies. Both antisera precipitated heavy and light chains of a MW of 70,000 and 24,000 daltons respectively. In addition, a band of 39,000 daltons was also evident. Reduction and alkylation of the iodinated molecule, followed by precipitation with anti-delta serum demonstrated only the 70,000 and 39,000 daltons bands. The present report indicates that by sensitive techniques, such as iodination and immunoprecipitation, non-reactive hidden light chains can be detected in IgD lambda molecules.

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Electrophoresis, Polyacrylamide Gel Immunoelectrophoresis Immunoglobulin D Immunoglobulin Light Chains Immunoglobulin lambda-Chains Molecular Weight Myeloma Proteins Journal Article Research Support, Non-U.S. Gov't

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G Spira, I Silvian, I Tatarsky, A Carter. 1983-05-27. Detection of IgD 'hidden' lambda light chain.. https://doi.org/10.1016/0022-1759(83)90348-4

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