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PubMed · 6412144

Functional identity between murine gamma interferon and macrophage activating factor.

R M Schultz·W J Kleinschmidt
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Abstract

We have previously suggested that two lymphokine activities-macrophage activating factor (MAF) and gamma interferon (IFN-gamma)-are mediated by the same molecule. Striking similarities were noted in their cellular biosynthesis, rate of inactivation with acid treatment and heating, and elution profiles on Sephacryl S-200. In addition, highly specific polyclonal antibodies to murine IFN-gamma neutralized MAF and IFN-gamma to a similar degree. However, definitive studies require a pure product and we now report that murine IFN-gamma that had been cloned and expressed in a simian nonlymphoid cell line shows MAF activity. But it is not yet known whether IFN-gamma is responsible for all the MAF activity in media conditioned by T cells as the possibility for MAF heterogeneity remains.

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AnimalsCytotoxicity, ImmunologicInterferon-gammaLymphokinesMacrophage ActivationMacrophage-Activating FactorsMacrophagesMiceJournal Article

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R M Schultz, W J Kleinschmidt. Functional identity between murine gamma interferon and macrophage activating factor.. https://doi.org/10.1038/305239a0

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