A Drosophila metabolic gene transcript is alternatively processed.

S Henikoff; J S Sloan; J D Kelly

doi:10.1016/0092-8674(83)90374-4

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A Drosophila metabolic gene transcript is alternatively processed.

S Henikoff·J S Sloan·J D Kelly

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Abstract

We have determined the organization and transcription of a Drosophila DNA segment coding for the purine pathway enzyme, GAR transformylase. Because the transcript encoding this enzyme is very rare, we used a novel method for exon mapping without isolating a cDNA. Our results suggest a long polypeptide having multiple domains, with GAR transformylase at the COOH terminus preceded by an extensive repeat. Part of the same DNA segment specifies a shorter transcript, which consists of the same 5' exons but lacks the last three 3' exons. A polyadenylation signal within an intron allows this single gene to encode two polypeptides. We hypothesize that alternative processing of a transcript is a basis for channeling metabolic intermediates into two different pathways.

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Acyltransferases Amino Acid Sequence Animals Base Sequence Chromosome Mapping DNA Drosophila melanogaster Hydroxymethyl and Formyl Transferases Phosphoribosylglycinamide Formyltransferase Transcription, Genetic Journal Article Research Support, U.S. Gov't, P.H.S.

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S Henikoff, J S Sloan, J D Kelly. 1983. A Drosophila metabolic gene transcript is alternatively processed.. https://doi.org/10.1016/0092-8674(83)90374-4

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