A non-dissociable rabbit IgG-protein a complex.

G Mota; C Galatiuc; J Sjöquist; V Ghetie

doi:10.1016/s0769-2625(83)80127-5

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A non-dissociable rabbit IgG-protein a complex.

G Mota·C Galatiuc·J Sjöquist·V Ghetie

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Abstract

Glutaraldehyde treatment of rabbit IgG antibody complexed with protein A of Staphylococcus aureus (SpA) enabled the complex (IgG2-SpA1)2 to maintain its molecular weight unchanged and to become non-dissociable at low pH and in excess of IgG. The glutaraldehyde-treated (IgG2-SpA1)2 complex had the same half-life, complement-activating capacity and ability to interact with the Fc receptor as the non-treated complex. Moreover, the glutaraldehyde-treated complex had a stronger immunosuppressive effect on the synthesis of anti-sheep red blood cell antibody than either the non-complexed antibody or the IgG antibody complexed with SpA but untreated with glutaraldehyde.

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Animals Antibody Affinity Binding Sites, Antibody Complement Fixation Tests Glutaral Hemagglutination Tests Immunoglobulin G Immunoglobulin M Immunosuppression Therapy Macromolecular Substances Rabbits Rosette Formation Staphylococcal Protein A Journal Article Research Support, Non-U.S. Gov't

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G Mota, C Galatiuc, J Sjöquist, V Ghetie. A non-dissociable rabbit IgG-protein a complex.. https://doi.org/10.1016/s0769-2625(83)80127-5

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