Fiberoptic fluorometer for skin flap assessment.

Abstract

The fiberoptic fluorometer permits quantitative measurement of fluorescence after intravenous administration of fluorescein. Accurate assessment of tissue fluorescence can be made at any time after the injection, and both uptake and elimination of fluorescein can be followed. The instrument provides instantaneous digital readout of the fluorescence measured in dye fluorescence (DF) units and an index predictive of viability called the dye fluorescence index (DFI). The unit is portable, can be sterilized for operating room use, and can be operated by paramedical personnel with ease. We have used the instrument extensively in clinical and laboratory investigations, where it has been highly reliable in predicting survival and necrosis of a wide variety of flaps. Little or no operating time is added, and serial, low-dose fluorescein injections may be used to monitor a flap in the recovery room or at the bedside in the early postoperative period.