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Simple and rapid method for isolating large plasmid DNA from lactic streptococci.

D G Anderson·L L McKay
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Abstract

A procedure for the rapid isolation of plasmid DNA larger than 30 megadaltons from lactic streptococci is described. This protocol can be used on a preparative scale to isolate sufficient quantities of plasmid DNA required for restriction analysis, cloning, or transformation experiments. A scaled-down protocol is very useful for rapidly screening the plasmid content of streptococcal strains. With this methodology, previously undetected large plasmids were observed.

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DNA, BacterialLactococcus lactisMethodsPlasmidsComparative StudyJournal ArticleResearch Support, Non-U.S. Gov't

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D G Anderson, L L McKay. 1983. Simple and rapid method for isolating large plasmid DNA from lactic streptococci.. https://doi.org/10.1128/aem.46.3.549-552.1983

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