Cellobiohydrolase from Trichoderma reesei.

M Nummi; M L Niku-Paavola; A Lappalainen; T M Enari; V Raunio

doi:10.1042/bj2150677

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Cellobiohydrolase from Trichoderma reesei.

M Nummi·M L Niku-Paavola·A Lappalainen·T M Enari·V Raunio

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Abstract

A 1,4-beta-D-glucan cellobiohydrolase (EC 3.2.1.91) was purified from the culture liquid of Trichoderma reesei by using biospecific sorption on amorphous cellulose and immunoaffinity chromatography. A single protein band in polyacrylamide-gel electrophoresis and one arc in immunoelectrophoresis corresponded to the enzyme activity. The Mr was 65 000. The pI was 4.2-3.6. The purified enzyme contained about 10% hexose. The enzyme differs from previously described cellobiohydrolases in being more effective in the hydrolysis of cellulose.

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Cellulose 1,4-beta-Cellobiosidase Electrophoresis, Polyacrylamide Gel Glycoside Hydrolases Hydrolysis Immunodiffusion Immunosorbent Techniques Mitosporic Fungi Polysaccharides Trichoderma Journal Article

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M Nummi, M L Niku-Paavola, A Lappalainen, T M Enari, V Raunio. 1983-12-01. Cellobiohydrolase from Trichoderma reesei.. https://doi.org/10.1042/bj2150677

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