Amino acid sequence diversity in mouse lambda 2 variable regions.

B W Elliott; H N Eisen; L A Steiner

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PubMed · 6434637

Amino acid sequence diversity in mouse lambda 2 variable regions.

B W Elliott·H N Eisen·L A Steiner

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Abstract

The lambda-chains of immunoglobulins from BALB/c mice constitute the simplest system presently available for studying patterns of variable-region diversity. The limited number of V lambda and J lambda germ-line gene segments facilitates comparison of expressed and germ-line sequences. We report here the complete amino acid sequence of the variable regions of three lambda 2 chains and of one chain representing a V lambda 2----J lambda 3 rearrangement. Together with the previously determined sequence of the lambda 2 chain from myeloma MOPC-315, the results illustrate the following types of variable-region diversification: expression of a single V gene segment with more than one J segment, variability at the V-J junction, and presumably, somatic mutation in V and in J. The extent of somatic diversification in these lambda 2 chains is limited, consistent with results obtained previously with lambda 1 chains.

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Amino Acid Sequence Animals Antibody Diversity Genetic Code Immunoglobulin J-Chains Immunoglobulin Light Chains Immunoglobulin Variable Region Immunoglobulin lambda-Chains Mice Mice, Inbred BALB C Peptide Fragments Trypsin Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

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B W Elliott, H N Eisen, L A Steiner. 1984. Amino acid sequence diversity in mouse lambda 2 variable regions.. https://pubmed.ncbi.nlm.nih.gov/6434637/

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