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PubMed · 6442362

Template-directed synthesis with 2-aminoadenosine.

K Grzeskowiak·T R Webb·L E Orgel
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Abstract

A random copolymer, poly(CA), containing approximately equal amounts of cytidine (C) and adenosine (A), when incubated with a mixture of guanosine-5'-phosphoro-(2-methylimidazole) (2-MeImpG) and uridine-5'-phosphoro-(2-methylimidazole) (2-MeImpU), facilitates the incorporation of uridine (U) into oligomeric products with low efficiency. If 2-aminoadenosine (aA) is substituted for adenosine in the template, U is incorporated into the products with much higher efficiency. Random copolymers of C and U act as templates for the efficient synthesis of oligomers from 2-MeImpG and 2-MeImpA only if the concentration of substrates is relatively high (0.1 M). The substitution of 2-MeImpA permits the reaction to occur with much lower substrate concentrations. This effect is most prominent for template containing large amounts of U.

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AdenosineBiological EvolutionOligonucleotidesPoly APoly CPoly UComparative StudyJournal ArticleResearch Support, U.S. Gov't, P.H.S.

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BibTeXRIS

K Grzeskowiak, T R Webb, L E Orgel. 1984. Template-directed synthesis with 2-aminoadenosine.. https://doi.org/10.1007/bf02100631

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