PubMed · 6444115
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M E Curtin. 1984. Genetic engineering: guidelines upgraded.. https://pubmed.ncbi.nlm.nih.gov/6444115/
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The potential of the filamentous fungus Trichoderma reesei for producing heterologous proteins has recently been demonstrated with a number of secreted proteins. Rate-limiting steps and ways of improving the production have been studied, especially using antibody Fab fragments. Major improvements have been achieved by producing the foreign protein fused to the fungal cellulase cellobiohydrolase I. In addition to the strong inducible cbh1 promoter, other promoters, which are regulated in a different manner, have been developed.
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