Complementation of a dnaC initiation defect in vitro.

S J Projan; J A Wechsler

doi:10.1007/bf00270141

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PubMed · 6460155

Complementation of a dnaC initiation defect in vitro.

S J Projan·J A Wechsler

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Abstract

The dnaC28 mutant, CT28-3b, is an initiation defective dnaC strain. Extracts of the mutant failed to synthesize DNA in vitro when the strain was incubated at the restrictive temperature for two generation times prior to preparation of the extract. Addition of a complementing extract from a Col-E1::dnaC+ hybrid plasmid containing strain or of partially purified dnaC protein resulted in substantial synthesis. Hybridization of the DNA made by these in vitro complementation extracts showed that a significant portion of this DNA was from the region near the chromosomal origin of replication.

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Bacteriophage lambda DNA Replication DNA, Bacterial DNA, Viral Escherichia coli Genetic Complementation Test Mutation Nucleic Acid Hybridization Phenotype Plasmids Recombination, Genetic Journal Article Research Support, U.S. Gov't, P.H.S.

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S J Projan, J A Wechsler. 1981. Complementation of a dnaC initiation defect in vitro.. https://doi.org/10.1007/bf00270141

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