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A method for eliminating Rayleigh scattering from fluorescence spectra.

D I Kells·J D O'Neil·T Hofmann
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Abstract

A numerical method is described for the elimination of Rayleigh scattering from protein fluorescence emission spectra. The method is based upon the observation that Rayleigh scattering is symmetrical about a wavelength at or near the wavelength of excitation. It works best when an automated, computer-based approach can be applied to spectra collected on a data-logging device, although manual correction of chart-recorded spectra is also possible.

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Calcium-Binding ProteinsChemical PhenomenaChemistryGlycogenLightScattering, RadiationSolventsSpectrometry, FluorescenceJournal Article

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BibTeXRIS

D I Kells, J D O'Neil, T Hofmann. 1984. A method for eliminating Rayleigh scattering from fluorescence spectra.. https://doi.org/10.1016/0003-2697(84)90010-1

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