Coagulation Factor V contains copper ion.

K G Mann; C M Lawler; G A Vehar; W R Church

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PubMed · 6490642

Coagulation Factor V contains copper ion.

K G Mann·C M Lawler·G A Vehar·W R Church

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Abstract

Preparations of bovine and human coagulation Factor V were analyzed for copper using both atomic absorption and atomic emission spectroscopy. All preparations of the bovine and human protein were found to contain copper ion at a ratio of 1 copper ion bound per mol (Mr = 330,000) of Factor V. As a result of copper binding and sequence homology between ceruloplasmin and Factor V, bovine Factor V and thrombin-activated Factor V (Va) were assessed with respect to their visible and near ultraviolet absorption spectra and to their ability to oxidize N,N-dimethyl-p-phenylenediamine (a substrate for ceruloplasmin). Factor V and Factor Va exhibited absorption spectra with no maxima at either 310 or 610 nm, indicating that the copper is not bound in a site analogous to Type I or Type III copper sites in ceruloplasmin. Further, Factor V and Factor Va are not capable of serving as catalysts for the oxidation of N,N-dimethyl-p-phenylenediamine under solution conditions that are optimum for ceruloplasmin oxidase activity. These data suggest that the copper ion bound to Factor V may be functionally and structurally distinct from the Type I and Type III copper ion bound to ceruloplasmin.

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Animals Binding Sites Cattle Copper Factor V Factor Va Humans Molecular Weight Protein Binding Species Specificity Spectrophotometry Spectrophotometry, Atomic Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

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K G Mann, C M Lawler, G A Vehar, W R Church. 1984-11-10. Coagulation Factor V contains copper ion.. https://pubmed.ncbi.nlm.nih.gov/6490642/

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