Immune adherence hemagglutination: alternative to complement-fixation serology.

E T Lennette; D A Lennette

doi:10.1128/jcm.7.3.282-285.1978

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Immune adherence hemagglutination: alternative to complement-fixation serology.

E T Lennette·D A Lennette

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Abstract

Immune adherence hemagglutination (IAHA) was compared to complement fixation (CF), using standard procedures, for serological testing of human sera with a number of commercially available antigens. The antigens included herpes simplex, measles, cytomegalo-, and influenza (type B) viruses, as well as Mycoplasma pneumoniae and Chlamydia psittaci (Chlamydia group). The IAHA test was found to be as specific as the CF test, but 4 to 20 times as sensitive with all antigens tested. Antigen titers were also higher with the IAHA method, and the time required to complete the test was only 4 h for the IAHA method, compared with 20 h for the CF method. The increased sensitivity of the IAHA test should permit its use for determination of immunity, as well as for serodiagnosis of recent infections.

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Antibodies, Bacterial Antibodies, Viral Chlamydia Infections Complement Fixation Tests Hemagglutination Tests Humans Immune Adherence Reaction Mycoplasma Infections Virus Diseases Comparative Study Journal Article Research Support, U.S. Gov't, P.H.S.

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E T Lennette, D A Lennette. 1978. Immune adherence hemagglutination: alternative to complement-fixation serology.. https://doi.org/10.1128/jcm.7.3.282-285.1978

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