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PubMed · 6497991

A simple method for isolating osteoblasts from mouse calvaria.

A Zambonin Zallone·A Teti
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Abstract

A method for isolating osteoblasts from mouse calvaria, based on the capability of these cells to migrate onto plastic substrates, is reported. The osteoblasts migrate from small (1-2 mm2) regularly cut fragments of calvaria directly onto plastic surface as a continuous cellular sheet. Isolated osteoblasts retain a poligonal shape and the ability to initiate bone matrix formation in presence of B-glycero phosphate. The method is simple and provides a large quantity of osteoblasts ready to be used directly without the need of being detached and reseeded.

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A Zambonin Zallone, A Teti. 1984-08-31. A simple method for isolating osteoblasts from mouse calvaria.. https://pubmed.ncbi.nlm.nih.gov/6497991/

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