Nucleosome reconstruction via phosphorus mapping.

G Harauz; F P Ottensmeyer

doi:10.1126/science.6505674

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PubMed · 6505674

Nucleosome reconstruction via phosphorus mapping.

G Harauz·F P Ottensmeyer

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Abstract

Electron spectroscopic imaging was combined with reconstruction algorithms to derive the three-dimensional structure of the nucleosome core particle to a resolution of 1.5 nanometers. Images of phosphorus distributions within individual nucleosomes were interpreted as projections of a supercoil of DNA. These were used to orient the corresponding individual nucleosome images, making it possible to reconstruct the entire nucleosome in three dimensions. The structure is consistent with known biochemical and biophysical data and explains site-specific nuclease sensitivity, although differing in part with other nucleosome models.

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DNA Deoxyribonucleoproteins Histones Microscopy, Electron Models, Structural Nucleic Acid Conformation Nucleosomes Protein Conformation Spectrum Analysis Journal Article Research Support, Non-U.S. Gov't

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G Harauz, F P Ottensmeyer. 1984-11-23. Nucleosome reconstruction via phosphorus mapping.. https://doi.org/10.1126/science.6505674

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