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PubMed · 6506141

Systematic comparison of three basic methods of sample pretreatment for high-performance liquid chromatographic analysis of antiepileptic drugs using gas chromatography as a reference method.

U Jürgens·T May·K Hillenkötter·B Rambeck
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Abstract

Sera of epileptic patients which were routinely examined by gas chromatography (GC) were also analysed using high-performance liquid chromatography (HPLC). Three basic methods for the pretreatment of samples for HPLC analysis were compared: protein precipitation by adding acetonitrile to the serum, direct serum extraction using ethylacetate, and partitioning of serum and buffer solution over a stationary phase and extraction with dichloromethane/2-propanol. The analytical performance and practicability of the three methods were tested under routine conditions. The following anti-epileptic drugs and metabolites were used in the comparison of HPLC with GC: ethosuximide, primidone, phenobarbital, phenytoin, carbamazepine, N-desmethylmethsuximide, and phenylethylmalonediamide.

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AnticonvulsantsCarbamazepineChromatography, GasChromatography, High Pressure LiquidCosts and Cost AnalysisHumansKineticsPhenytoinReference ValuesComparative StudyJournal Article

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U Jürgens, T May, K Hillenkötter, B Rambeck. 1984. Systematic comparison of three basic methods of sample pretreatment for high-performance liquid chromatographic analysis of antiepileptic drugs using gas chromatography as a reference method.. https://doi.org/10.1097/00007691-198409000-00014

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