A pilot experiment for the micronucleus test. The multi-sampling at multi-dose levels method.

M Hayashi; T Sofuni; M Ishidate

doi:10.1016/0165-7992(84)90091-5

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A pilot experiment for the micronucleus test. The multi-sampling at multi-dose levels method.

M Hayashi·T Sofuni·M Ishidate

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Abstract

A pilot experiment was undertaken to find the optimal dose range and sampling time in the micronucleus test; 2 animals per group were used. The chemical was injected at 4 different doses, and smear preparations were made from femoral marrow cells at 5 different sampling times for each dose group. The incidence of micronucleated polychromatic erythrocytes was scored on the preparations stained with acridine orange. When the data were arranged two-dimensionally, the optimal dose and sampling time which could be used for a further full-scale test, could be estimated. In addition, sex differences and the effect of multiple treatment can be estimated using this protocol with minor modifications.

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Animals Cell Nucleus Erythrocytes Female Male Mice Mice, Inbred Strains Mutagenicity Tests Mutagens Mutation Sex Factors Journal Article

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M Hayashi, T Sofuni, M Ishidate. A pilot experiment for the micronucleus test. The multi-sampling at multi-dose levels method.. https://doi.org/10.1016/0165-7992(84)90091-5

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