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A second site-specific restriction endonuclease from Staphylococcus aureus.

J S Sussenbach·P H Steenbergh·J A Rost·W J van Leeuwen·J D van Embden
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Abstract

A site-specific restriction endonuclease has been isolated from Staphylococcus aureus PS 96. This enzyme, Sau96 I, recognizes the DNA sequence 5'--G-G-N-C-C--3' and cleaves as indicated by the arrows. The enzyme 3'--C-C-N-G-G--5' cleaves adenovirus type 5 and lambda DNA many times, SV40 DNA 10 times and 0X174 RF DNA 2 times. Evidence is presented that the enzyme is involved in biological restriction-modification.

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Base SequenceBinding SitesDNA, ViralEndonucleasesStaphylococcus aureusSubstrate SpecificityJournal Article

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J S Sussenbach, P H Steenbergh, J A Rost, W J van Leeuwen, J D van Embden. 1978. A second site-specific restriction endonuclease from Staphylococcus aureus.. https://doi.org/10.1093/nar%2F5.4.1153

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