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PubMed · 6525373

[DNA-cholesterol complex studied by fluorescent probes].

M G Shengeliia·M M Gachava·M A Tsartsidze·B A Lomsadze
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Abstract

DNA interaction with cholesterol at various lipid concentrations has been investigated by the fluorescent probes method. It has been shown that the intensity of acridine orange fluorescence in the DNA-cholesterol complex decreases at 24 micrograms/ml cholesterol and at 45 micrograms/ml it increases. The number of binding sites and the degree of polarization of fluorescence change simultaneously. Binary mechanism of cholesterol binding with DNA has been suggested: surface binding takes place at low concentrations, intercalation--at high lipid concentrations.

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Acridine OrangeAnimalsBinding SitesCattleCholesterolDNADose-Response Relationship, DrugFluorescent DyesIn Vitro TechniquesSolutionsSpectrometry, FluorescenceEnglish AbstractJournal Article

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M G Shengeliia, M M Gachava, M A Tsartsidze, B A Lomsadze. 1984. [DNA-cholesterol complex studied by fluorescent probes].. https://pubmed.ncbi.nlm.nih.gov/6525373/

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