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Nucleosome mass distribution using image averaging.

C L Woodcock·J Frank
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Abstract

Scanning transmission electron microscopy has been used to obtain mass distribution data for unstained, randomly oriented dinucleosomes. These data were then subjected to computer analysis to increase the signal/noise ratio, and define the major sources of image variation within the population. Average images of groups of similar particles have a mass distribution consistent with current models of nucleosome structure, and a resolution approaching 3 nm. The potential value of this method for analyzing structural variants of nucleosomes is discussed.

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AnimalsChickensErythrocytesMicroscopy, Electron, ScanningNucleosomesJournal Article

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C L Woodcock, J Frank. 1984. Nucleosome mass distribution using image averaging.. https://doi.org/10.1016/s0022-5320(84)80045-3

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