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Quantitation of complement factor D in human serum by a solid-phase radioimmunoassay.

S R Barnum·M A Niemann·J F Kearney·J E Volanakis
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Abstract

A sensitive solid-phase radioimmunoassay is described which quantitates human D to 1-2 ng/ml. The assay was used to measure the concentration of D in normal and acute-phase sera and sera from individuals with systemic lupus erythematosus. All 3 groups of sera had comparable levels of D with mean values of 1.8, 2.3 and 2.5 micrograms/ml, respectively. Also tested were sera decomplemented in vitro by activators of the classical and alternative pathways. The results indicated that D is not depleted by alternative or classical pathway activation. However, heat inactivation (56 degrees C, 30 min) of serum resulted in almost complete loss of antigenic D.

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C-Reactive ProteinComplement Activating EnzymesComplement ActivationComplement Factor DHemolysisHumansLupus Erythematosus, SystemicRadioimmunoassayJournal ArticleResearch Support, U.S. Gov't, P.H.S.

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S R Barnum, M A Niemann, J F Kearney, J E Volanakis. 1984-03-16. Quantitation of complement factor D in human serum by a solid-phase radioimmunoassay.. https://doi.org/10.1016/0022-1759(84)90470-8

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