High-performance liquid chromatographic separations on dihydroxyboryl-agarose.

S Hjertén; D Yang

doi:10.1016/s0021-9673(00)96161-2

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High-performance liquid chromatographic separations on dihydroxyboryl-agarose.

S Hjertén·D Yang

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Abstract

This paper (which is part of a series of articles on high-performance liquid chromatographic separations on agarose columns) describes the chromatographic behaviour of catecholamines, nucleosides, aminoacylated and non-aminoacylated tRNA, and glycosylated and non-glycosylated hemoglobins on agarose matrices derivatized with m-aminophenylboronic acid. These matrices primarily bind molecules with two vicinal hydroxy groups in the cis-configuration. The possible existence of secondary electrostatic and hydrophobic interactions has been studied with the aid of diagrams showing retention time as a function of pH and buffer concentration.

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Boronic Acids Catecholamines Chromatography, High Pressure Liquid Glycated Hemoglobin Humans Nucleosides RNA, Transfer RNA, Transfer, Amino Acyl Sepharose Journal Article Research Support, Non-U.S. Gov't

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S Hjertén, D Yang. 1984-12-21. High-performance liquid chromatographic separations on dihydroxyboryl-agarose.. https://doi.org/10.1016/s0021-9673(00)96161-2

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