Human leukemic cells contain transforming growth factor.

H Nakamura; K Komatsu; H Akedo; M Hosokawa; H Shibata; T Masaoka

doi:10.1016/0304-3835(83)90199-4

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Human leukemic cells contain transforming growth factor.

H Nakamura·K Komatsu·H Akedo·M Hosokawa·H Shibata·T Masaoka

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Abstract

A transforming growth factor was found in the extracts of leukemic cells obtained from the peripheral blood of 11 patients with leukemia. This factor stimulated the colony formation of anchorage-dependent BALB/c 3T3 cells in soft agar. The high levels of colony-stimulating activities were observed in the cell extracts from patients with chronic myelogenous leukemia in blastic crisis (CML BC), acute myelogenous leukemia and CML in chronic phase. The factor from a CML BC patient was heat- and acid-labile, relatively stable to dithiothreitol treatment and inactivated by pronase treatment. Molecular size of the factor seems more than 10,000 daltons.

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Acids Agar Animals Cell Extracts Cell Line Cell Separation Dithiothreitol Hot Temperature Humans Leukemia Leukocytes Mice Molecular Weight Peptides Transforming Growth Factors Comparative Study Journal Article Research Support, Non-U.S. Gov't

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H Nakamura, K Komatsu, H Akedo, M Hosokawa, H Shibata, T Masaoka. 1983. Human leukemic cells contain transforming growth factor.. https://doi.org/10.1016/0304-3835(83)90199-4

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