Nude mice as models for human leukemia studies.

G K Potter; R N Shen; J W Chiao

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PubMed · 6582788

Nude mice as models for human leukemia studies.

G K Potter·R N Shen·J W Chiao

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Abstract

Human leukemic promyelocytes of the HL-60 line were grown as tumors in nude mice and studied. A single nonmetastatic granulocytic sarcoma developed after subcutaneous inoculation with HL-60 cells. Some exceeded 5 cm in size. Almost all mice developed tumors after initial priming with cyclophosphamide. Older sarcomas showed viable tumor islands in necrotic and fibrotic tissue. Some tumors appeared greenish. Histologic and electron-microscopic analysis demonstrated large, vaguely outlined cells in poorly vascularized sheets. The cells displayed high nucleocytoplasmic ratios, basophilic granular cytoplasms lacking Auer rods, and enzymes characteristic of cultured HL-60 promyelocytes. Some tumor cells also demonstrated monocyte/macrophage enzymes, such as butyrate esterase. Induced HL-60 tumors also corresponded morphologically to a human subcutaneous promyelocytic tumor specimen. Comparative cytologic studies of induced HL-60 tumors in nude mice and cultured HL-60 cells revealed virtual identity, suggesting the nude mouse tumor as a useful model for in vivo studies of human leukemic cells.

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Animals Cell Line Cell Transformation, Neoplastic Disease Models, Animal Glucosephosphate Dehydrogenase Histocytochemistry Humans Leukemia, Myeloid, Acute Mice Mice, Nude Neoplasm Transplantation Sarcoma, Experimental Journal Article Research Support, U.S. Gov't, P.H.S.

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G K Potter, R N Shen, J W Chiao. 1984. Nude mice as models for human leukemia studies.. https://pubmed.ncbi.nlm.nih.gov/6582788/

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