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PubMed · 6606487

New bone resorption stimulation factor elaborated by a human osteosarcoma cell line.

G Eilon·C Trummel·B Kream·M V Viola
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Abstract

The FM-2 cell line is a cloned, immortalized cell line derived from a human osteosarcoma. Conditioned medium from FM-2 cultures contains a factor which stimulates calcium mobilization from fetal rat bone organ cultures. Treated bones contain increased numbers of osteoclasts and decreased bone matrix. This factor has a molecular weight of approximately 29,000 as determined by gel filtration. Its biological activity is dependent on a protein moiety and is completely inhibited by calcitonin. Its synthesis by the FM-2 line is dependent on cell density and replenishment of fresh medium. This factor is not parathyroid hormone, a vitamin D metabolite, prostaglandin E, epidermal growth factor, or osteoclast-activating factor, all of which have bone-resorbing activities. Also, FM-2-conditioned medium inhibits collagen synthesis in fetal rat calvaria cells and decreases alkaline phosphatase levels in an osteoblastic cell line, and these two properties coelute with the calcium-mobilizing factor from a hydroxylapatite column. These biological products, synthesized by a cell line derived from a tumor, may represent physiological factors normally synthesized by a subpopulation of bone cells.

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AnimalsBiological AssayBiological ProductsBone and BonesCalciumCell LineCells, CulturedClone CellsCytokinesEpidermal Growth FactorFemaleFetusHumansMiceOsteosarcomaPregnancyJournal ArticleResearch Support, Non-U.S. Gov't

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G Eilon, C Trummel, B Kream, M V Viola. 1984. New bone resorption stimulation factor elaborated by a human osteosarcoma cell line.. https://pubmed.ncbi.nlm.nih.gov/6606487/

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