Quantitative analyses of complement fixation in three immune complex systems.

C Horgan; R P Taylor

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PubMed · 6611301

Quantitative analyses of complement fixation in three immune complex systems.

C Horgan·R P Taylor

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Abstract

The quantitative interaction of three different immune complex systems with complement has been investigated. dsDNA/anti-DNA, heat-aggregated IgG subfractions, and human IgG/rabbit anti-human IgG complexes were tested for their ability to consume complement (immune haemolysis assay) and to bind to red blood cells in a complement-mediated reaction (the RBC-CF assay). Our results indicate that some physical and immunological properties of the dsDNA/anti-DNA immune complex systems are significantly different from those of immune complexes that involve more common globular protein antigens. This difference in properties may help explain the role of dsDNA/anti-DNA immune complexes in systemic lupus erythematosus pathogenesis.

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Animals Antibodies, Antinuclear Antigen-Antibody Complex Complement Fixation Tests Complement System Proteins Dose-Response Relationship, Immunologic Hot Temperature Humans Immunoglobulin G Protein Denaturation Rabbits Solubility Journal Article Research Support, U.S. Gov't, P.H.S.

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C Horgan, R P Taylor. 1984. Quantitative analyses of complement fixation in three immune complex systems.. https://pubmed.ncbi.nlm.nih.gov/6611301/

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