Protein loss during nuclear isolation.

P L Paine; C F Austerberry; L J Desjarlais; S B Horowitz

doi:10.1083/jcb.97.4.1240

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Protein loss during nuclear isolation.

P L Paine·C F Austerberry·L J Desjarlais·S B Horowitz

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Abstract

Cryomicrodissection makes possible the measurement of the entire in vivo protein content of the amphibian oocyte nucleus and provides a heretofore missing baseline for estimating protein loss during nuclear isolation by other methods. When oocyte nuclei are isolated into an aqueous medium, they lose 95% of their protein with a half-time of 250 s. This result implies an even more rapid loss of protein from aqueously isolated nuclei of ordinary-size cells.

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Animals Cell Fractionation Cell Nucleus Female Oocytes Proteins Xenopus Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

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P L Paine, C F Austerberry, L J Desjarlais, S B Horowitz. 1983. Protein loss during nuclear isolation.. https://doi.org/10.1083/jcb.97.4.1240

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