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Light scattering measurements supporting helical structures for chromatin in solution.

A M Campbell·R I Cotter·J F Pardon
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Abstract

Laser light scattering measurements have been made on a series of polynucleosomes containing from 50 to 150 nucleosomes. Radii of gyration have been determined as a function of polynucleosome length for different ionic strength solutions. The results suggest that at low ionic strength the chromatin adopts a loosely helical structure rather than a random coil. The helix becomes more regular on increasing the ionic strength, the dimension resembling those proposed by Finch and Klug for their solenoid model.

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AnimalsCell NucleusChickensChromatinErythrocytesLightNucleic Acid ConformationProtein ConformationScattering, RadiationJournal Article

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A M Campbell, R I Cotter, J F Pardon. 1978. Light scattering measurements supporting helical structures for chromatin in solution.. https://doi.org/10.1093/nar%2F5.5.1571

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