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Gradient optimization principles in reversed-phase high-performance liquid chromatography and the separation of influenza virus components.

M A Phelan·K A Cohen
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Abstract

Reversed-phase high-performance liquid chromatography is used to separate the major proteins of A/Bangkok/1/79 X 73 influenza virus. The purity of the isolated proteins, their yield and reactivity with monoclonal antibodies are discussed. The virus, in turn, is used as a probe to examine some theoretical gradient principles relating resolution, retention time and gradient time. Empirical compliance with these principles is generally shown.

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Chromatography, High Pressure LiquidOrthomyxoviridaeViral ProteinsJournal Article

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M A Phelan, K A Cohen. 1983-08-26. Gradient optimization principles in reversed-phase high-performance liquid chromatography and the separation of influenza virus components.. https://doi.org/10.1016/s0021-9673(01)90879-9

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