A discontinuous electrophoretic system for separating peptides on polyacrylamide gels.

J Kyte; H Rodriguez

doi:10.1016/0003-2697(83)90118-5

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A discontinuous electrophoretic system for separating peptides on polyacrylamide gels.

J Kyte·H Rodriguez

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Abstract

An electrophoretic system for separating, with high resolution, peptides 25-250 residues in length is described. The peptides are stacked by discontinuous electrophoresis to form very sharp bands at the origin. They are then separated on a matrix of 20% polyacrylamide, 8 M urea, and 0.1% dodecyl sulfate. Through this combination, high resolution and clean separation, based on polymer length, are achieved.

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Animals Electrophoresis, Polyacrylamide Gel Peptide Fragments Peptides Sodium Dodecyl Sulfate Urea Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.Research Support, U.S. Gov't, P.H.S.

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J Kyte, H Rodriguez. 1983. A discontinuous electrophoretic system for separating peptides on polyacrylamide gels.. https://doi.org/10.1016/0003-2697(83)90118-5

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