Particle size distribution analysis as a rapid method to detect significant bacteriuria.

J S Baker; G M Ederer; D Mundschenk

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PubMed · 6650557

Particle size distribution analysis as a rapid method to detect significant bacteriuria.

J S Baker·G M Ederer·D Mundschenk

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Abstract

Particle size distribution analysis (PSDA) was evaluated as a rapid screening method for detecting significant bacteriuria by linking a C1000 Channelyzer to a Coulter Counter. Colony counts and PSDA screening results were compared for 800 urine specimens. The PSDA method proved to be 92% specific, but only 64% sensitive, for detecting at least 10(5) colony forming units (CFU)/ml. By performing serial dilution colony counts on 109 culture-positive specimens (greater than or equal to 10(5) CFU/ml), it was determined that the low level of sensitivity (64%) was due to culture-positive specimens that had between 10(5) and less than 10(7) CFU/ml. The sensitivity of the PSDA method increased to 90% and 100% when culture-positive specimens had 10(7) or more and 10(8) or more CFU/ml, respectively. The low level of sensitivity demonstrated in this investigation makes the introduction of this method into clinical usage unacceptable at this time.

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J S Baker, G M Ederer, D Mundschenk. 1983. Particle size distribution analysis as a rapid method to detect significant bacteriuria.. https://pubmed.ncbi.nlm.nih.gov/6650557/

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