A structural characterization of gap junctions isolated from mouse liver.

S S Sikerwar; S K Malhotra

doi:10.1016/0309-1651(83)90208-4

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A structural characterization of gap junctions isolated from mouse liver.

S S Sikerwar·S K Malhotra

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Abstract

Mouse liver gap junctions have been isolated by using an anionic detergent, n-dodecanoyl sarcosine, in combination with non-ionic polyoxyethylene ethers (Brij 35 and Brij 58) and (W-1) detergents. Purified gap junctions are obtained in a sucrose step gradient containing 1-o-n-octyl-beta-D-glucopyranoside. This procedure is aimed at minimizing proteolysis. The junctions thus isolated have a hexagonal lattice of connexons with a lattice constant of 7.6-8.4 nm. Presence of a major Mr 26,000 gap junctional protein has been confirmed by SDS-PAGE.

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Animals Cell Fractionation Centrifugation, Density Gradient Intercellular Junctions Liver Mice Microscopy, Electron Journal Article Research Support, Non-U.S. Gov't

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S S Sikerwar, S K Malhotra. 1983. A structural characterization of gap junctions isolated from mouse liver.. https://doi.org/10.1016/0309-1651(83)90208-4

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