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An affinity ligand for phospholipase A2 purification.

S Tahir·R C Hider
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Abstract

An alkyl ether analog of phosphatidylcholine was synthesized and used as a ligand to purify acid-extracted phospholipase A2 from bovine ileum smooth muscle by affinity chromatography in the presence of cholate. This ligand contains a primary amino group at the omega-position of the acyl chain in position 1 and so permits direct covalent coupling with the ester group of Affi-Gel-10. An endogenous membrane bound phospholipase A2 has been purified 32-fold in a good yield (70%) employing this ligand in an affinity chromatography step.

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AnimalsCattleChromatography, AffinityIleumLigandsPhospholipasesPhospholipases APhospholipases A2Journal Article

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S Tahir, R C Hider. 1983. An affinity ligand for phospholipase A2 purification.. https://doi.org/10.1016/0003-2697(83)90691-7

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