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Exodeoxyribonuclease from rat brain specific for single-stranded DNA.

V A Ivanov·A I Gaziev·T M Tretyak
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Abstract

An exodeoxynuclease with single-stranded DNA specificity has been isolated from rat brain and purified to electrophoretic homogeneity. Approximately 1100-fold purification with a yield of 16% has been achieved by chromatography on DNA-agarose, hydroxyapatite and Sephadex G-200. The enzyme has a pH optimum at 8.4, requires Mg2+ or Mn2+ as a cofactor and has a molecular weight of about 60000. It hydrolyzes homologous, heterologous, synthetic and depurinated substrates at the same rate liberating nucleoside 5'-monophosphates but does not attack ultraviolet-irradiated polydeoxyribonucleotides. This DNase is localized predominantly in neuronal cell nuclei and appears to be lacking in rat liver tissue.

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AnimalsBrainCell NucleusChemical PhenomenaChemistryDNA, Single-StrandedExodeoxyribonucleasesHydrolysisNeuronsRatsSolubilitySubstrate SpecificityJournal Article

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V A Ivanov, A I Gaziev, T M Tretyak. 1983-12-15. Exodeoxyribonuclease from rat brain specific for single-stranded DNA.. https://doi.org/10.1111/j.1432-1033.1983.tb07856.x

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