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Calcium-induced changes in thyroglobulin conformation.

S Formisano·B Di Jeso·R Acquaviva·E Consiglio·G Palumbo
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Abstract

Polyethylene glycol solutions (10% w/v) were used to detect the effect of mono- and divalent cations on some properties of thyroglobulin. It is shown that in presence of 10% w/v polyethylene glycol in 0.01 M Tris-HCl, pH 7.5, calcium (less than 0.05 M) modifies the solubility, the sedimentation rate, and the Stokes' radius of thyroglobulin, while monovalent cations up to 0.6 M do not effect any of these properties. These findings can be explained by an increase in molecular compactness of thyroglobulin. Furthermore, it was shown that a synthetic polymer, polyethylene glycol, could be used to detect conformational changes.

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AnimalsCalciumCattleCentrifugation, Density GradientChromatography, GelPolyethylene GlycolsProtein BindingProtein ConformationSolubilityThyroglobulinThyroid GlandJournal ArticleResearch Support, U.S. Gov't, P.H.S.

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S Formisano, B Di Jeso, R Acquaviva, E Consiglio, G Palumbo. 1983. Calcium-induced changes in thyroglobulin conformation.. https://doi.org/10.1016/0003-9861(83)90463-0

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