PubMed · 6674295
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G Gee. 1983. Infrared flash synchronization.. https://pubmed.ncbi.nlm.nih.gov/6674295/
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Distribution of millimeter wavelength electromagnetic energy absorption in surface layers of biological tissue models was studied using methods of Infrared Thermography. 0.1 mm thin-layer phantoms were irradiated in the near field using different types of horn antennas in the 37-78 GHz frequency range. Heating patterns were recorded during microwave irradiation, and surface SAR distributions were calculated. The temperature resolution was better than 0.05 K. It was found that horn antennas produced nonuniform heating patterns in irradiated objects. These nonuniform patterns were due to a geometrical resonance resulting from a secondary wave-mode interaction between an irradiated object and the corresponding critical cross-section of the horn antenna. Local SAR values in hot spots exceeded the spatially averaged values by over 10 times, and the widths of these hot spots at 5 times the average SAR were often 1 mm or less. The location, quantity, number and size of the local field absorption maxima of irradiated objects strongly depended on the frequency of electromagnetic irradiation, with equivalent Q-factors of 500 or more. These findings provide an explanation for a number of frequency-dependent effects of millimeter wave electromagnetic irradiation.
Individual yeast cells can be successfully isolated and recultured on plates with a new isolation method making use of optical trapping with infrared laser light. The cells can be selected on morphological criteria by high resolution microscopy. The isolation device is constructed from two coverslips separated by spacers, in which selected cells are transferred to a plastic capillary, using the optical trap. To test the procedure, selection experiments were done with a mixture of two Saccharomyces cerevisiae strains, distinguishable both in fluorescence microscopy and on agar plates. These experiments showed that only selected cells were isolated, and close to 100% of the isolated stationary-phase cells formed colonies on agar plates, indicating a high recovery. A lower recovery was obtained with exponential-phase cells, possibly because of a higher sensitivity to laser irradiation. Applications for this method may include the isolation of mutants with altered morphology and the isolation of subpopulations of yeast cultures, for their separate investigation or for the initiation of pure cultures.
Applied infrared photography has changed significantly during the last 40 years. Its medical application has dwindled while its overall use has increased dramatically. An understanding of the electromagnetic spectrum and the relationship between what humans are capable of seeing and what infrared sensitive film can record will lead to a better appreciation of this film's potential application in today's world. Improvements in processing have resulted in greater film speed and the direct conversion of the film to positive transparencies. This latter development will aid individuals who use the film as an educational teaching aid.