Correlation between segmental flexibility and effector function of antibodies.

V T Oi; T M Vuong; R Hardy; J Reidler; J Dangle; L A Herzenberg; L Stryer

doi:10.1038/307136a0

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Correlation between segmental flexibility and effector function of antibodies.

V T Oi·T M Vuong·R Hardy·J Reidler·J Dangle·L A Herzenberg·L Stryer

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Abstract

Mouse monoclonal anti-dansyl antibodies with the same antigen-binding sites but different heavy chain constant regions were generated. The extent of segmental flexibility in times of nanoseconds and the capacity to fix complement were greatest for IgG2b, intermediate for IgG2a, and least for IgG1 and IgE. Hence, the effector functions of immunoglobulin isotypes may be controlled in part by the freedom of movement of their Fab arms.

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Animals Antibodies Binding Sites, Antibody Complement Activation Immunoglobulin E Immunoglobulin G Mice Motion Protein Conformation Spectrometry, Fluorescence Structure-Activity Relationship Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

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V T Oi, T M Vuong, R Hardy, J Reidler, J Dangle, L A Herzenberg, L Stryer. Correlation between segmental flexibility and effector function of antibodies.. https://doi.org/10.1038/307136a0

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