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PubMed · 6692859

Electrofusion. A new, highly efficient technique for generating somatic cell hybrids.

C Finaz·A Lefevre·J Teissié
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Abstract

A rapid and highly efficient method for generating viable somatic cell hybrids is described. A co-culture of clone ID and CH rodent cell lines was exposed to five successive electric pulses of about 1.5 kV/cm with a duration of 50 musec. This treatment induced extensive cell fusion, and independent hybrid clones were generated with a frequency of 1 X 10(-3), which represents a 100-fold increase over the polyethylene glycol induced fusion. Seventeen of them were propagated in selective medium, karyotyped and analysed for their enzyme markers, in order to establish their hybrid nature.

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AnimalsCell FusionCell LineCricetinaeElectricityHybrid CellsIsoenzymesKaryotypingMethodsMiceJournal ArticleResearch Support, Non-U.S. Gov't

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C Finaz, A Lefevre, J Teissié. 1984. Electrofusion. A new, highly efficient technique for generating somatic cell hybrids.. https://doi.org/10.1016/0014-4827(84)90592-5

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