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Phosphate depletion decrease mitogen-mediated stimulation of phospholipid synthesis in human peripheral lymphocytes.

C Rampini·C Dubois·V Barbu
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Abstract

Concanavalin A-mediated stimulation of 32P-phosphate incorporation into phospholipids of human peripheral lymphocytes is comparatively studied in normal and phosphate-depleted media. In the phosphate-depleted medium, 2 hours after the start of cell activation, the stimulation sharply decreases for phosphatidylinositol (6.5-fold) and for phosphatidylcholine (in the latter case, the stimulation is even replaced by a slight inhibition of the incorporation). These results must be related to the rate-limiting effect of inorganic phosphate on ATP formation and thus on phospholipid synthesis, an effect which may be particularly pronounced when there is both phosphate depletion and cell activation.

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HumansKineticsLymphocytesPhosphatesPhospholipidsPhosphorus RadioisotopesJournal Article

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C Rampini, C Dubois, V Barbu. 1984-01-13. Phosphate depletion decrease mitogen-mediated stimulation of phospholipid synthesis in human peripheral lymphocytes.. https://doi.org/10.1016/0006-291x(84)91111-2

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