Monitoring yeast spindles in the fluorescence microscope.

C Baumstark-Khan; H Rink; H P Zimmermann

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PubMed · 6698040

Monitoring yeast spindles in the fluorescence microscope.

C Baumstark-Khan·H Rink·H P Zimmermann

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Abstract

Formation of the complete spindles during the budding process of Saccharomyces uvarum was investigated by fluorescence microscopy of protoplasted cells. Protoplasts were treated with anti-tubulin antibodies and DAPI, a fluorescent dye staining DNA. Thus, both chromatin and spindles could be visualized. Duplication as well as formation of separated spindle pole bodies during the different stages of budding are documented, demonstrating the occurrence and behaviour of microtubules during yeast cell cycle.

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Antibodies Cell Cycle Microscopy, Fluorescence Saccharomyces Spindle Apparatus Tubulin Journal Article

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C Baumstark-Khan, H Rink, H P Zimmermann. 1984. Monitoring yeast spindles in the fluorescence microscope.. https://pubmed.ncbi.nlm.nih.gov/6698040/

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