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Interrelationships among distinct idiotypic specificities.

S T Ju·M Pierres·M Fougereau·M E Dorf
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Abstract

The fine idiotypic properties of a hybridoma (H51.85.2) anti-GAT antibody which lacks both CGAT and GA-1 idiotypes are described. We identified another idiotype, termed GA-2 idiotype, on H51.85.2 hybridoma antibody. The GA-2 idiotype is present in all mouse strains tested and is induced by GA-related antigenic determinants. An interesting relationship between GA-1 and GA-2 idiotype on hybridoma anti-GA antibodies was observed. All GA-1+ hybridomas also express GA-2 idiotype. In contrast, GA-2+ hybridomas can express a full set, a fraction, or none of the GA-1 idiotypic determinants. The data together with previous amino acid sequence studies strongly suggest that H51.85.2 hybridoma antibody was derived from the same VH and VL genes encoding the GA-1+ hybridoma antibodies.

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Amino Acid SequenceAnimalsAntibodies, MonoclonalAntibody SpecificityHybridomasImmunoglobulin IdiotypesMiceMice, Inbred StrainsPeptidesPolymersJournal Article

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S T Ju, M Pierres, M Fougereau, M E Dorf. Interrelationships among distinct idiotypic specificities.. https://doi.org/10.1016/s0769-2625(84)80013-6

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