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PubMed · 6716367

A simplified procedure for separating amniotic fluid phospholipids with thin-layer chromatography.

J M Dugan·G Knee·J F Strauss·J C Touchstone
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Abstract

A simple, unidimensional, thin-layer-chromatographic separation of amniotic fluid phospholipids involving detection with a phosphorus-specific spray was compared to the two-dimensional, thin-layer-chromatographic method described by Gluck et al. There were close correlations between the two techniques for lecithin/sphingomyelin ratios and for the presence of phosphatidylglycerol. The advantages of the unidimensional method are a shorter procedure time, the ability to analyze multiple samples on a single plate and the simplicity of a single-solvent system.

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AmniocentesisAmniotic FluidChromatography, Thin LayerEvaluation Studies as TopicFemaleHumansPhosphatidylcholinesPhospholipidsPregnancySphingomyelinsComparative StudyJournal Article

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J M Dugan, G Knee, J F Strauss, J C Touchstone. 1984. A simplified procedure for separating amniotic fluid phospholipids with thin-layer chromatography.. https://pubmed.ncbi.nlm.nih.gov/6716367/

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