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PubMed · 6721993

A rapid method for determination of acetylation phenotype using dapsone.

P A Philip·M S Roberts·H J Rogers
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Abstract

A rapid, simple one-stage protein precipitation method for the estimation of plasma dapsone (DDS) and monoacetyldapsone (MADDS) concentration by high-pressure liquid chromatography (h.p.l.c.) is described. Its performance in the assignment of acetylator phenotype was compared with a reference h.p.l.c. method utilising an extraction procedure and internal standard. The rapid h.p.l.c. technique combined with measurement of the plasma MADDS/DDS ratio is, in our opinion, the method of choice for the determination of the acetylator phenotype in population studies.

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AcetylationChemistry, OrganicChromatography, High Pressure LiquidDapsoneHumansOrganic Chemistry PhenomenaPhenotypeJournal ArticleResearch Support, Non-U.S. Gov't

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P A Philip, M S Roberts, H J Rogers. 1984. A rapid method for determination of acetylation phenotype using dapsone.. https://doi.org/10.1111/j.1365-2125.1984.tb02373.x

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