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An automatic pulsed laser microfluorometer with high spatial and temporal resolution.

F Docchio·R Ramponi·C A Sacchi·G Bottiroli·I Freitas
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Abstract

The paper describes an automatic pulsed laser microfluorometer with high spatial and temporal resolution, developed in our laboratories. The instrument consists of: (i) a nitrogen-laser-pumped dye-laser for the excitation of the fluorescence, (ii) a microscope with additional optics to focus the excitation beam on the sample and to collect the fluorescence, (iii) filters or monochromators to select the output wavelength, (iv) a fast photomultiplier tube to detect the signal, and (v) a dual time-scale microprocessor-controlled signal averager for the acquisition and processing of the signal. Examples are given that show the potential of the time-resolved fluorescence microscopy in studying, quantitatively and qualitatively, the properties of fluorescent molecules.

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AnimalsBase CompositionChromatinDNAHumansLasersLymphocytesMicroscopy, FluorescencePlant CellsJournal Article

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F Docchio, R Ramponi, C A Sacchi, G Bottiroli, I Freitas. 1984. An automatic pulsed laser microfluorometer with high spatial and temporal resolution.. https://doi.org/10.1111/j.1365-2818.1984.tb02504.x

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