Membrane associated proteins and malignancy: an experimental hypothesis.

B Mély-Goubert; M H Freedman

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PubMed · 6777032

Membrane associated proteins and malignancy: an experimental hypothesis.

B Mély-Goubert·M H Freedman

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Abstract

Normal human lymphocytes and human tumor cell lines were labeled with an apolar fluorescent probe (1, 6-diphenyl-1, 3, 5-hexatriene), and fluoresence polarization (P) values were determined. The arguments presented in this study suggest that although P values represent an intricate average of all labeled hydrophobic regions of the cell (phospholipid bilayers, membrane proteins and enzymes, etc.), to a large extent, interactions of the probe with membrane proteins are of primary importance. The lower P values obtained with the tumor cell lines, compared with the normal lymphocytes, were interpreted as indicating alterations in either the structure or concentration of a membrane associated protein(s).

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Animals Calcimycin Cell Membrane Diphenylhexatriene Humans Leukemia Lymphocytes Membrane Proteins Mice Mice, Inbred CBA Neoplasm Proteins Neoplasms Spectrometry, Fluorescence Journal Article

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B Mély-Goubert, M H Freedman. 1980. Membrane associated proteins and malignancy: an experimental hypothesis.. https://pubmed.ncbi.nlm.nih.gov/6777032/

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