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PubMed · 6792060

Terminal stages of complement hemolysis: technical comment.

G H Wirtz·S S Westfall
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Abstract

In studying terminal lytic events of sensitized erythrocytes which have reacted with all 9 components of complement, we agree in general with the conclusions of Boyle and Borsos [1] that the cellular intermediate passes through the stages E*bound, E*inserted, and E*doomed. However, we differ with these workers on the influence of 3 variables on the terminal reactions. These variables are: time of hemolysis; temperature of E* preparation; and the use of EDTA to manipulate E*.

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AnimalsComplement C8Complement C9Complement System ProteinsEdetic AcidErythrocytesGuinea PigsHemolysisImmunologic TechniquesTemperatureTime FactorsJournal ArticleResearch Support, Non-U.S. Gov'tResearch Support, U.S. Gov't, P.H.S.

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G H Wirtz, S S Westfall. 1981. Terminal stages of complement hemolysis: technical comment.. https://doi.org/10.1016/0165-2478(81)90095-x

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