PubMed Health⌕ Search
Home/Archive/PubMed

PubMed · 6792500

New immunodiagnostic methods.

S Krakowka
View original source ↗Export metadata ↓

Abstract

A quiet revolution in the field of immunodiagnosis is underway. Its objective is to provide simple and accurate methods for making a laboratory diagnosis of infectious diseases, endocrinopathies, and immunodeficiencies. Monoclonal antibody technology will provide unlimited quantities of antisera with defined and stable antigenic specificity. These antibodies will be incorporated into new serologic methods, namely enzyme-linked immunosorbent assay and agglutination tests. Unlike traditional methods, these new procedures are easily adaptable to kit form and consequently will likely become available for routine use in clinical situations in the near future.

Explore related subjects

Agglutination TestsAnimalsEnzyme-Linked Immunosorbent AssayImmune SeraImmunodiffusionImmunologic TechniquesInfectionsJournal Article

Keep this discovery

Explore connections, maps & timelines

BibTeXRIS

S Krakowka. 1981. New immunodiagnostic methods.. https://pubmed.ncbi.nlm.nih.gov/6792500/

Cite the original work for its findings. Save a collection to share your selection of sources.

KEEP EXPLORING

Related citations

PubMed1995

Automated reading and processing of quantitative IgG, IgM, IgA, and IgE isotypic agglutination results in microplates. Development and application in parasitology-mycology.

Microplate agglutination techniques represent a simple and commonly used approach for the quantitative or qualitative isotypic analysis of specific antibodies. However, they require optical reading by the investigator and are thus prone to an important degree of variability. In order to solve some of the problems associated with the variability of optical readings, we have used an automatic reader scanning each of the 96 wells of a standard microplate in 32 different locations. The inherent advantages of the automatic reader were further maximized by coupling it to a dedicated computer running customized software designed to process data coming on-line from the spectrophotometer. This approach has been applied to the diagnosis of human toxoplasmosis and candidosis. Suspensions of Toxoplasma gondii tachyzoites or of sensitised erythrocytes were used for the determination of IgG antibodies or the quantification of IgM, IgA, or IgE specific isotypes. This procedure allows the simple and reproducible collection of objective results. Moreover, it permits a reduction in cut-off values and direct interpretation of results with automatic conversion of scores into titer, units, index, or into any other scale appropriate for standardization purposes.

Agglutination Tests↗
PubMed1995

Human lymphocyte activation is associated with the early and high-level expression of the endogenous lectin CSL at the cell surface.

Lymphocytes undergo activation in response to antigens, cytokines, lectins and antibodies interacting with specific cell-surface molecules or through substances influencing signal transduction pathways. This study shows that human T- and B-cells stimulated using phorbol esters or plant lectins express early (2 h using phorbol esters and 24 h using plant lectins) a high level of a polyvalent carbohydrate-binding protein, the cerebellar soluble lectin (CSL), which is in part externalized. The lectin, immunologically related to CDw70, interacts with specific glycoprotein ligands of the lymphocyte surface, including CD3 on T-cells and CD24 on B-cells. Major changes in phosphorylations associated with activation appear as largely CSL-dependent since they are specifically inhibited by anti-CSL Fab fragments. It is suggested that the lectin induces the clustering of specific cell-surface glycoproteins and plays the role of an endogenous amplifier of activation signals.

Agglutination Tests↗